HLA-DRB1 Shared Epitope RA Risk Panel
PCR panel targeting key HLA-DRB1 markers associated with rheumatoid arthritis risk and severity, including Shared Epitope motifs, Val11, and DRB1*09:01.
Ambient shipping and storage 
Single reaction wells format
High accuracy and sensitivity 
Automation-ready Product Description
The Hola HLA-DRB1 Shared Epitope Lyo PCR Kit is a lyophilized real-time PCR panel under development for qualitative analysis of selected HLA-DRB1 markers relevant to rheumatoid arthritis.
The Shared Epitope (SE) refers to amino acid motifs such as QKRAA and QRRAA encoded by the HLA-DRB1 gene. It is one of the strongest known genetic risk factors for rheumatoid arthritis, particularly for ACPA-positive disease, which is associated with faster progression and structural damage.
The panel further includes Valine at position 11 (Val11), associated with erosion risk, and HLA-DRB1*09:01, a marker relevant in selected Asian populations.
The kit is being developed in a single-reaction lyophilized PCR strip format for use with selected open qPCR instruments and Hola MDX systems.
Technical Specifications
- Product status: In development
- Panel type: HLA-DRB1 Shared Epitope
- Marker coverage: Shared Epitope-associated HLA-DRB1 motifs, Val11, HLA-DRB1*09:01
- Technology: Real-time PCR-based design
- Sample type: Blood / DNA extracted from blood
- Reaction format: Single-reaction well format in PCR strip
- Compatible instruments: Bio-Rad CFX96 / CFX Opus, Hola MDX01, Hola MDX08
- Storage and transport: +2 °C to +40 °C
- Intended use: Research use only
Each Kit Contains
- Lyophilized MasterMix in pre-aliquoted strip wells
- Lyophilized Positive Control
- Negative Control
Frequently Asked Questions
What is the HLA-DRB1 Shared Epitope PCR Kit used for?
- The kit is being developed for qualitative analysis of selected HLA-DRB1 markers, including Shared Epitope-associated motifs, Val11, and HLA-DRB1*09:01.
Which markers are included in the panel?
- The planned panel includes Shared Epitope-associated HLA-DRB1 motifs such as QKRAA and QRRAA, Valine at position 11 (Val11), and HLA-DRB1*09:01.
Which sample type is required?
- The intended sample type is blood. The PCR workflow is designed for analysis of DNA extracted from blood samples.
Which instruments are compatible?
- The kit is being developed for compatibility with Bio-Rad CFX96 / CFX Opus, Hola MDX01, and Hola MDX08 systems.
What format will the kit use?
- The kit is being developed in a single-reaction lyophilized PCR strip format with pre-aliquoted MasterMix.
