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Bordetella Lyo PCR Kit

Producer: Hola Biolabs s.r.o.

Lyophilized Real-Time PCR kit for the detection of Bordetella pertussis and Bordetella parapertussis.

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Description
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Product description

The Hola Bordetella Lyo PCR Kit enables highly sensitive detection of Bordetella pertussis and Bordetella parapertussis bacteria using IS481 and IS1001 multicopy sequences. For unambiguous determination, one of the targets of the reaction is also the gene for the pertussis toxin PtxA.

Technical specifications

  • Single reaction wells format (PCR strip)
  • Recommended instruments: CFX96/CFX Opus (Bio-Rad), Hola MDX01, Hola MDX08
  • Research use only
  • Endogenous internal control
  • Three-channel multiplex PCR
  • Sample type: sputum, upper respiratory tract samples (nasal cavity, nasopharynx)
  • Specificity: 100%
  • Sensitivity (LoD): 0.1 copies/μL (IS481), 0.7 copies/μL (IS1001), 5.9 copies/μL (ptxA-Pr)
  • Storage and transport at a temperature of +15 °C to +25 °C

The kit contains

  • Lyophilized MasterMix in strip wells
  • Lyophilized Positive control
  • Negative control
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